LC-MS/MS and tandem MS/MS identification of chemical compounds of the stem bark of Acacia macrostachya (Mimosaceae)

Abstract

The main objective of this work was to identify chemical compounds of biological interest in the methanol extract of Acacia macrostachya stem bark using High-Performance Liquid Chromatography coupled with tandem Mass Spectrometry (HPLC-MS/MS) and Global Natural Product Social Molecular Networking (GNPS) databases. A total of seven (07) molecules from four families of secondary metabolites such as Kaempferol-3-O-rutinoside, kaempferol, epicatechin, epicatechin-3-O-gallate, bergaptol-O-β-D-glucopyranoside, 3-hydroxyurs-12-en-28-oic acid and kaempferid were identified The presence of these compounds in the extract could be responsible of the anti-diabetic and anti-cancer properties of this extract which were reported in our previous investigations.