Evaluation of CYP3A4 activity and tetracycline residues in meat poultry after exposure to Hydroxytyrosol, Anethole and Eugenol

Abstract

Background: The residues of antibiotics in meat especially in chicken meat are a risk source on public health in animal and human. This study aimed to determine tetracycline residues in the chicken samples (thigh, chest and liver) via analytical method HPLC with evaluation of CYP3A4 in liver tissue homogenate using Eliza Kit after administration of Hydroxytyrosol, Anethole and Eugenol. Methods : In this study, sixty chickens chosen according to weight and healthy condition divided into four equal groups, they dosed the antibiotic tetracycline (250 mg / liter) for 3 days. First group as positive control second received orally eugenol (150 mg/kg), third group received orally anethol (100 mg/kg), and fourth group received orally hydroxytyrosol (50 mg/kg). These compounds were administrated only on the 4th and 5th day of the experiment, on the 6th day all poultry were slaughtered and samples were taken from the thigh, liver and chest to evaluate the tetracycline residues. Results: The present study noted that hydroxyltyrosol has moderate activity to clear the tetracycline residual from chicken thigh, chest and liver without effect on CYP3A4 activiy, a significant induction of CYP3A4 activity was recorded with using of eugenol and anethole that might be was responsible of metabolism and excretion of tetracycline residues.