Bio-synthesis and biopotential assessment of silver nanoparticles (AgNPs) by using leaf extract of Ocimum sanctum

Abstract

Silver nanoparticles (AgNPs) have great potential for their potential role in biomedical researches. In the present study AgNPs have been synthesized utilizing in vitro grown leaf extract of Ocimum sanctum by the reduction of silver nitrate solution. The green synthesized AgNPs were partialy characterized by UV-visible spectroscopy with maximum absorbance (λmax) at 427nm. The Fourier transform infrared spectroscopy (FTIR) was carried out to identify the possible biomolecules responsible for efficient stabilization of AgNPs. Green synthesised AgNPs seemed to exhibit capacity to work as good antioxidant agent (a potent radical scavenger properties by 2, 2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging assay) and have antibacterial activity against E. coli bacterial strain in present study. Our results explain that Ocimum sanctum leaf extract have potential as bio-reducing agent for synthesis of AgNPs, which can be exploited as anti-oxidant, antimicrobial agent and depicting an effective way for utilizing bioactive resources in restoration of medicinal properties of this plant Ocimum sanctum with high efficacy.