Persea americana seed and Bryophyllum pinnatum leaf ethyl acetate binary combinations protects against non-steroidal anti-inflammatory drug-induced oxidative stress

Abstract

The present study investigated the protective effect of binary mixtures of ethyl acetate fractions of Persea americana seed (PAEA) and Bryophyllum pinnatum leaf (BPEA) against non-steroidal anti-inflammatory drug (NSAID) induced oxidative stress in rats. The study comprised of 10 experimental units of four (4) rats each. The control groups were normal control group (NC), untreated control (UC), standard (0.02g kg-1 body weight Omeprazole (OMEP). Treatment groups included single and binary combinations of PAEA and BPEA, PAEA+ BPEA (1:1), PAEA+ BPEA (1:2), PAEA+ BPEA (1:3), PAEA+ BPEA (2:1), PAEA+ BPEA (3:1). The rats were initially administered 0.4gKg-1 b.wt. of respective mixtures orally for 3 weeks. Oxidative stress was induced by administration of 0.03 g/kg body weight indomethacin on the 22nd day after overnight fasting. Oxidative markers were determined using reference methods. The results revealed that indomethacin administration resulted in increased malondialdehyde level and significant reduction in stomach tissues GSH concentration when matched with normal control rats. Gastric tissue SOD, Catalase, GST, and GPx activity were significantly (p PAEA + BPEA (1:1), > PAEA + BPEA (1:2) > PAEA + BPEA (1:3) > PAEA > BPEA.