Phytochemical, antioxidant and proximate analysis of Berberis aristata roots

Abstract

Berberis aristata or Tree Turmeric is a shrub native to the Himalayan region and it has been used in traditional medicine for centuries. The present study aimed to determine the phytochemical profile, antioxidant potential, and proximate composition of Berberis aristata roots. Aqueous root extract was prepared and analyzed for bioactive components using standard phytochemical screening methods. Quantitative analysis revealed significant total phenolic and flavonoid content. The extract exhibited dose-dependent antioxidant activity in (2,2-Diphenyl-1-Picrylhydrazyl) DPPH and (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) ABTS radical scavenging assays. The IC50 values of 57.874 µg/ml was obtained for DPPH radical scavenging assay and IC50 values of 58.04 µg/ml was obtained for ABTS radical scavenging assay. Ferric Reducing Antioxidant Power (FRAP) was also evaluated and the activity was found to be 52.932±0.0181 Fe (II) iron equivalent (µM). Proximate analysis of the root powder determined moisture, ash, acid-insoluble ash, ether extract, and crude protein content. Results indicate that Berberis aristata roots contain a diverse array of phytochemicals with notable antioxidant properties, supporting its traditional medicinal uses and potential for modern pharmaceutical applications. The study provides a comprehensive characterization of Berberis aristata root extract, contributing to the scientific understanding of this important medicinal plant.