Scientific validation of secondary metabolites profile from stem part of medicinally important plant Vincetoxicum subramanii (AN Henry) Meve & Liede (Apocynaceae) using TLC and HPLC method

Abstract

Secondary compounds of V. subramanii stem were qualitatively analyzed using TLC and HPLC. Ethanol stem extracts were prepared for qualitative determination of secondary metabolites. The resulting extracts were subjected to a variety of solvent-based systems, using conventional TLC techniques to identify secondary metabolites, followed by HPLC analysis to identify commonly available compounds such as quercetin and catechins. including alkaloids, flavonoids, phenols, tannins, saponins and glycosides. It became clear that chromatograms generated at different retention times show the presence of different plant constituents as a result of HPLC analysis. 19 peaks were identified. V. subramanii stem were found to contain alkaloids, flavonoids, glycosides, saponins and steroids.