Detection and biological characterization of Cucumber mosaic virus on chilli (Capsicum annuum) in Pothia block of Kishanganj

Abstract

Within the family Bromoviridae, the genus Cucumovirus includes the rapidly spreading plant pathogen known as Cucumber mosaic virus (CMV), which affects over 1200 species across 100 families of monocot and dicot plants. Chilli is highly susceptible to CMV, which can result in a variety of symptoms as mottling and yellowing of the leaves, and ultimately the distortion of the chilli leaf and fruit, which can cause significant financial losses. In the current investigation, we have gathered the chilli leaves exhibiting the typical CMV symptoms from the Pothia block of Kishanganj, specifically from Kharkhari, Motihara, Ganyabari, Lodhabari, and Arrabari. The CMV was identified using the Dot immunobinding assay and the direct antigen coating-enzyme linked immunosorbent assay (DAC-ELISA of 165 suspicious samples collected, 47 were found to be positive for the CMV antisera. Remaining 118 field samples tested negative for CMV antisera, which may be affected with other biotic and abiotic stresses. Incidence of Cucumber mosaic virus was varies in between 20% to 33% against CMV antiserum in and around of the Pothia block of Kishanganj.