Development of in vitro protocol for Khaya senegalensis

Abstract

Studies were carried out during 2023, for the development of in vitro protocol for Khaya senegalensis. Among the media of MS and MS media with growth regulators, the medium of MS with kinetin was found to be optimal and ideal compared to other media with 22.05% Morphogenic response (%). Among the explants collected, explants from Nodal segments showed greater response compared to the explants from shoot tips and axillary buds. But the explants rom shoot tips showed good response at earlier stages while the response became slower later. Compared to MS basal medium, treatment with MS +5.0 mg/l Kinetin was found to be best treatment. The best treatments for obtaining shoot induction was MS medium supplemented with MS + 7.0 mg/l BAP recorded highest per cent shoot induction (27.50%), number of shoots per explants (3.00) and average shoot length (1.92 cm). Among the various concentration of kinetin investigated MS + 5.0 mg /l Kinetin showed highest per cent shoot induction with (32.50%) number of shoots per ex-plant (4.00) and highest average shoot length (2.90cm). The shoots subcultured in MS medium supplemented with auxins such as IBA and IAA did not induce rhizogenesis.