Establishment of protocol for in vitro propagation of Bambusa nutans using ex-plant from nursery raised plant

Abstract

Bambusa nutans, a species of bamboo known for its ornamental and economic value, faces challenges in propagation due to slow growth rates and limited seed availability. In this study, we aimed to establish a protocol for in-vitro propagation of B. nutans using ex-plant derived from nursery-raised plants of Bambusa nutans. Nodal ex-plants obtained from B. nutans were sterilized and cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of plant growth regulators (PGRs), including cytokinins and auxins. The cultures were maintained under controlled environmental conditions with regard to temperature, light intensity, and photoperiod. After a period of culture, the responses of ex-plants to different PGR treatments were evaluated in terms of shoot proliferation, root induction, and overall growth performance. Our results demonstrate that the optimal protocol for in-vitro propagation of B. nutans involves the use of nodal ex-plants from nursery-raised plants of B. vulgaris cultured on MS medium supplemented with [insert specific PGR concentrations]. This protocol resulted in efficient shoot proliferation and root induction, with a high percentage of ex-plants exhibiting healthy growth. The established protocol offers a promising approach for mass propagation of B. nutans, which could contribute to conservation efforts and commercial cultivation of this valuable bamboo species.