Curvularia lunata causing leaf blight barnyard millet (Echinochloa frumentacea (Roxb.) link in India and its management by botanicals

Abstract

The management of leaf blight pathogen with nineteen plant extracts tested under in vitro conditions. Among twenty plant extracts the 5v (Vilvum, Vembu, Vanni, Vagai and Vengai) plants extracts (10%) effectively inhibited mycelium growth (84.81,64.77,63. 26,61.36 and 48.56 percent reduction over control) of Leaf blight pathogens. The fungus was further identified by amplifying internal transcribed spacer region sequence DNA extraction was carried out by scraping the mycelium and reproductive structures formed in monocultures of the Curvularia sp. DNA extraction was carried out according to the CTAB-based protocol, To amplify the DNA from the isolates, primers ITS1 (forward: 5’- TCCGTAGGTGAACCTGCGG-3’) and ITS4 (reverse: 5’TCCTCCGCTTATTGATATGC-3’). The resulting 550-bp sequence was submitted to GenBank with Accession No. OP604146.