New techniques in liquid chromatography for biomarker discovery

Abstract

Liquid chromatography (LC) has evolved significantly, becoming an indispensable tool for biomarker discovery due to its high resolution, sensitivity, and versatility. This review provides an in-depth look at recent advancements in liquid chromatography techniques, focusing on their applications in biomarker discovery. We explore innovations such as ultra-high-performance liquid chromatography (UHPLC), multidimensional chromatography, and hyphenated techniques, discussing their impact on enhancing analytical performance and their contributions to identifying novel biomarkers. We also address the challenges and future directions in this rapidly evolving field.