Studies on detrimental effect of cryopreservation on sperm progressive motility, viability, plasma membrane integrity and oxidative stress in Frieswal bull semen

Abstract

This experiment was designed to study the effect of cryopreservation on post-thaw sperm quality and oxidative stress in Frieswal bull semen. For this purpose, 18 ejaculates were collected from three Frieswal bulls using artificial vagina at biweekly intervals. The semen sample which possesses more than 70% progressive motility and above 600 million/ml spermatozoa concentration was subsequently subjected to a process for LN¬2 vapour freezing. Semen samples were extended in the GEYT extender. At the fresh stage, semen was evaluated for percent live spermatozoa, percent progressive motility, percent intact acrosome, and percent HOST reactive spermatozoa. At the post-thaw stage, semen was evaluated for percent progressive motility, percent live spermatozoa, percent intact acrosome, percent HOST reactive spermatozoa. Post-thaw semen, showed a significant (p