A promising study on cytotoxicity and in vitro anti-inflammatory activity of Heracleum candolleanum (Wight et. Arn.) gamble

Abstract

The methanol extracts of roots, seeds, and leaves of Heracleum candolleanum (Wight et. Arn.) Gamble were subjected to in vitro anti-inflammatory and anticancer studies. The anti-inflammatory studies were carried out using HRBC membrane stabilization, inhibition of protein denaturation, and proteinase inhibitory methods. Cytotoxicity of the extracts was evaluated on cancer cells of various origins, namely, A431, A375, MDA-MB-231, U937, and HeLa, representing non-melanoma, melanoma, breast, acute myeloid leukemia, and cervical cancer cell lines respectively. From the present study it can be concluded that methanol extract of all the three parts of H. candolleanum may have significant anti-inflammatory activity against inflammatory based diseases or allied conditions. The study also revealed that the roots, leaves and seed extract possessed selective in vitro cytotoxicity, against five different human malignant cell lines.