Newcastle disease virus detection and characterization using molecular biology techniques

Abstract

The identification of ND (Newcastle disease virus) by polymerase chain reaction examination completes the methodology of diagnosis of the disease, through remarkable sensitivity and speed in generating results. Also, the analyzing of the cleft sites of the fusion's gene (F gene), at the level of component nucleotides and subsequently their translation into specific encoded amino acids, provides important data on the pathogenicity degree of isolated virus strains. Thus, molecular biology techniques complement the diagnosis and characterization of Newcastle disease strains, being successfully used for herd surveillance. The study consisted of molecular analysis of (47) virus strains isolated between 2016 and 2022, of the vaccine strains used to combat the disease as well as of the pathogenic strain with the sign