Confirmation of F1 hybridity in upland cotton (Gossypium hirsutum) by molecular assay

Abstract

The F1 population was developed by crossing two species of Upland Cotton (Gossypium hirsutum). During the experiment, ICAR-CICR Rajat Bt had insect resistance, good adaptability, high yielding was choosen as a donor parent and a recently released non-bt variety Suvarna Shubra (AKH-09-5) that was resistant to bacterial leaf blight, grey mildew, Myrothecium leaf spot, and jassids was used as a female parent. The F1 hybrids exhibiting insect resistance were confirmed through PCR amplification by utilization of a SSR molecular marker. After screening 60 F1 plants, the cry1Ac gene was confirmed in 20 F1 hybrids. These hybrids might be an important genetic resource to carry on the bollgard resistance gene to further advanced generations.