Morphological characterisation of Colletotrichum lindemuthianum and In vitro efficacy of Bioagents: Insights from dual culture method

Abstract

The current study dealing with morphological characterization of Colletotrichum lindemuthianum and in vitro efficacy of various bioagents against the said pathogen by adopting the standard procedure of dual culture method was carried out. Morphological characterization revealed that the mycelium is hyaline to pale brown, branched with intercellular and intracellular septations. The conidia measured 9.87-11.30 × 3.43-4.19 μm (av. 9.98 × 4.11 μm) on host tissue and 11.76-21.44 × 3.88-5.94 μm (av. 16.73 × 4.32 μm) on potato dextrose agar media. The shape of conidia recorded was cylindrical with obtuse ends. Shape of acervulus observed under microscope was saucer like with dimensions ranging from 137-315 μm (av. 198.25 μm). The colour of conidia, acervuli and setae recorded was hyaline, black and brown to dark brown, respectively. No septation was observed in conidia or acervuli but setae had 0-3 septum. Among bio-agents tested in vitro Trichoderma viride proved to be the best with mean mycelial growth inhibition percentage of 69.44, followed by Trichoderma harzianum (63.88%), Psuedomonas flourescens (46.60%) and Bacillus subtilis (54.01%).