Purification and characterization of bioactive compounds extracted from (Hoom) Miliusa tomentosa

Abstract

Introduction: Green plant possesses highly diversified list of natural phytochemicals which are known to have wide range of bioactivities. These phytochemicals can be good sources for the discovery of new drugs. In this study, we report the partial phytochemical characterization and antimicrobial activities of a semi-purified fraction isolated from the Miliusa tomentosa plant belongs to family Annonaceae. Materials: The plant was taxonomically identified and leaves were collected at Umbalebailu, Bhadra Wildlife Sanctuary, Shivamogga, Karnataka, India. Methods: Extraction of bioactive molecules by using Chloroform for 10 hours by magnetic stirrer. The solvent extracted sample was subjected to phytochemicals analysis followed by purification by column chromatography. The separated fractions were confirmed by TLC and purified fractions were elevated for anti-oxidant and antimicrobial activities. Results: All the three fractions have sugars and flavonoids, However, Fraction-3 has glycosides and terpenoids and other phytochemicals. Column purified fractions were exhibited significant amount of antioxidant and antibacterial activity. Conclusion: The present findings suggest that the column purified fraction possesses effective antioxidant antimicrobial activities. Further studies are required to unveil its potential for application in pharmaceutical as well as food processing industries.