Molecular detection of john cunningham virus (JCV) in patients with colorectal cancer in khartoum, Sudan

Abstract

Background: Colorectal cancer (CRC) is the most repetitious malignancies with high mortality worldwide. JC virus (JCV) is ubiquitous Polyomavirus, with seroprevalence rates ranging from 70% to 90% in adult population. Recently the roles of JCV have been reported in many malignant tumors worldwide. The association of JCV was reported in patients with colon and rectum cancers Objective: the study was conducted to evaluate the association of JCV DNA in patients with colorectal cancer in Khartoum State. Materials and methods: A total of 70 formalin-fixed paraffin-embedded tissue block samples were collected including 33 (47.1%) male 37 (52.9%) female patients with CRC. DNA was extracted from all the samples. Nested PCR was carried out for detection of Vp1/T Ag junction genome in JCV genome by Nested-PCR assay. Results: 3/70(4.2%) samples of patients were positive for JCV DNA (P 0.522). Out of 3 samples positive for JC DNA, 2(2.8%) were males and 1(1.4%) was female. Conclusion: prevalence of JCV DNA was 4.2% among patients with CRC. Including 2.8% male and 1.4% female (p= 0.522). The subsequent T-Ag protein expression might explain the increased risk of colorectal cancer and requires further investigation