Ethanol production analysis in postmortem liver and muscle tissues of rats

Abstract

This study investigated ethanol production in postmortem liver and muscle tissues of rats. The analysis aimed to assess the presence and quantity of ethanol in these tissues after death. A total of 100 rats were included in the study, and their liver and muscle tissues were subjected to ethanol analysis using gas chromatography-mass spectrometry (GC-MS). The results revealed varying levels of ethanol production in both liver and muscle tissues, with ethanol detected in 65% of liver tissue samples and 45% of muscle tissue samples. The concentration of ethanol ranged from 0.05 mg/g to 0.2 mg/g in liver tissue and from 0.03 mg/g to 0.15 mg/g in muscle tissue. Additionally, the time course of ethanol production postmortem showed a gradual increase in ethanol levels up to 24 hours postmortem. These findings contribute to our understanding of ethanol metabolism in postmortem tissues and have implications for forensic investigations involving alcohol-related deaths.