Characterization of bacterial surface leached silica using 3D profilometry: Insights and Applications

Abstract

Four isolates of rhizobacteria that promote plant growth were effectively isolated from the rhizospheric zone of the soil. Among the four isolates, Bacillus licheniformis stood out for having the extraordinary capacity to reabsorb a significant amount of silica onto the surface. A key component in promoting plant growth is silica, which is well known for its functions in controlling nutrient intake, lowering metal toxicity, offering structural support, and assisting in photosynthesis. Using the precursor Di Magnesium Trisilicate, Bacillus licheniformis demonstrated the ability to synthesize 14 mg of silica in a 50-ml culture. Upon further investigation using a 3D optical profilometer, the extracted silica exhibited a 7.904 µm complex three dimensional structure and 8.06 µm surface roughness. The unique properties of Bacillus licheniformis and its ability to generate a significant quantity of silica, position this organism as an excellent candidate for biofertilizer development. The incorporation of the silica precursor highlights its potential as a biofertilizer and emphasizes how it can promote plant growth by providing better structural support and nutrient management.