Effect of black cumin (Nigella sativa) seeds extract in tris egg yolk citrate extender on sperm motility of cryopreserved Surti buck semen

Abstract

The present research work was conducted to examine effect of different concentrations of Black cumin (Nigella sativa) seeds extract in Tris egg yolk citrate extender on motility and viability of cryopreserved Surti buck semen. Total 64 semen ejaculates were collected from four Surti buck (16 ejaculates/buck) twice a week by artificial vagina method. Semen samples were diluted with Tris egg yolk citrate extender and Black cumin (Nigella sativa) seeds aqueous extract was supplemented at different concentrations viz. 0% (T1), 3% (T2), 4% (T3), 5% (T4) (pH 6.5-6.8) maintaining final concentration of 100×106sperm/ml. After making the groups, the semen samples were examined for individual sperm motility (%), Motility Degeneration Rate (MDR) (%) for all groups. Result showed that highest mean individual sperm motility (%) was found at initial, pre-freeze and post-thaw stage in T3 group followed by T2, T4 and T1 group. Lowest mean motility degradation rate (%) was found at pre-freeze and post-thaw stage in T3 group followed by T2, T4 and T1 group. It was concluded that post-thawed individual sperm motility and MDR parameters were well maintained at 4% supplementation of Black cumin (Nigella sativa) seed extract in Tris egg yolk citrate extender.