Molecular detection and sequence analysis of envelope glycoproteins (gC, gD) and tegument protein (UL47) of infectious laryngotracheitis virus isolated from commercial poultry flocks in Tamil Nadu, India

Abstract

Infectious laryngotracheitis is a respiratory tract infection of chickens. There is re-emergence of ILT especially in intensive poultry production region in Tamil Nadu causing severe economic loss. Vaccination is the best way to control ILT. The rise in ILTV infections have been correlated with use of live attenuated vaccines. This study has been attempted to understand the genetic variation in ILTV genes coding for immune dominant glycoproteins and tegument protein as candidates for next generation safer vaccines. The full-length amplification and sequencing of gC, gD and UL47 genes of an Indian isolate of ILTV has been recorded for the first time in India and accessioned in Gen Bank. This study revealed more than 99% identity matrix with published isolates from different countries, proving their conserved nature and possibility to use as effective vaccine candidate. The phylogenetic tree analysis depicts that the Indian ILTV sequences clustered with major reference sequences of the world.