Effect of total phenolic content (TPC) and activity of phenylalanine ammonia-lyase (PAL) on spot blotch of wheat incited by Bipolaris sorokiniana

Abstract

Spot blotch, caused by Bipolaris sorokiniana, is a devastating disease of wheat (Triticum spp.) in warm, humid regions of the world. Used to explain the role of total phenolic content (TPC) and phenylalanine ammoniacalase (PAL) activity. Total phenolic content of wheat leaves depending on cultivars, inoculation at different time intervals after inoculation The total phenolic content during inoculation was (120. 78 mg/gm) and it significantly increased as time increased from 24 to 48 hai and decreased suddenly at 72 hai. The yield and TPC value of 121.6 and 121.8 for their leaf phenolic content were observed in cultivars V1 and V2. It was equivalent to each other. Significantly more phenolic content 130.81 was observed in inoculated plants than non-inoculated plants, which gave the lowest phenolic content in wheat leaves. The total phenolic content at different hours after inoculation was 120.78 and it increases significantly when the time increases from 24 to 48 hours after inoculation and decreases after 72 hours after inoculation. PAL activity at challenge was (0.194 units/g) and increased significantly over time from 24 to 48 hai and decreased sharply in 72 hai.