Imidacloprid induced histopathological changes in kidney and ameliorative role of Withania somnifera in female rats

Abstract

In present experiment, the histopathological alterations in kidney caused due to oral administration of imidacloprid were studied and role of Withania somnifera in alleviating the renal damage was estimated. The female rats were used in the study were forty eight and they were grouped into 4 categories. Group 1 was control, Group 2 was imidacloprid control (30mg/Kg Body weight/Day/Oral), Group 3 was Withania somnifera control (1g/Kg feed) and Group 4 was treated with both imidacloprid and Withania somnifera. The kidney tissue samples were collected at the end of experiment to analyze the renal function. A significant (p<0.05) reduction in activities of glutathione, super oxide dismutase and a significant (p<0.05) elevation in thio barbituric acid reactive substances levels were observed in kidneys of imidacloprid treated rats. Microscopically, significant pathological changes were noticed in renal sections of group 2 rats whereas Withania somnifera as a nephroprotective molecule significantly reduced the renal damage and restored the renal function on imidacloprid induced nephrotoxicity.