Evaluation of Collagen Building, UV Protective and Antioxidant Properties of CollaBZen on Human Collagen Type I,II and Type IV

Abstract

CollaBZenTM from Zenherb labs, a proprietary blend of polyphenols extracted from Phyllanthus emblica, Camellia sinensis, and Coffea arabica, was assessed for its anti-ageing potential. Human dermal fibroblast and chondrocyte cells were exposed in vitro to hydrogen peroxide and UVA to induce ageing manifested as ROS production and collagen breakdown respectively. CollaBZenTM revealed no cytotoxicity up to 0.16 mg/ml in both NHDF and HCH cells. DCFDA assay revealed that CollaBZenTM demonstrated a dose-dependent reduction in H2O2-induced ROS levels in both cell types. Exposure to UVA rays led to collagen depletion but CollaBZenTM pre-treatment showed higher collagen levels, offering protection. At 0.04 mg/ml, it conferred a significant shield against UVA rays, with a 48.13%, 39.47%, and 30.5% increase in COL1A1, COL4A1, and COL2A1, respectively. Beyond promoting collagen as documented in the previous studies, CollaBZenTM emerges as a potent food supplement safeguarding skin and joint cells from ageing consequences, reinforcing its role in age-related preventive care.