Estimation of lovastatin from Pleurotus species by thin layer chromatography and spectrophotometer

Abstract

In the global market for oyster mushrooms (Pleurotus species) play a significant role. In many countries, several species are grown for food on both a large and small commercial scale. In this experiment, the determination of lovastatin in Pleurotus spp. were screened including strains namely, P. djamor, P. florida, P. ostreatus, and P. sajor-caju. Lovastatin was produced in solid-state fermentation as rice straw and PD broth. We observed that the presence of lovastatin at Rf value is 0.94 to 0.97 by thin layer chromatography (TLC) from different Pleurotus species and found that higher lovastatin concentration obtained from P. ostreatus at mycelial stage followed by P. florida at pinhead stage, the concentration of lovastatin is (545±21.3mcg/g) and (485±21.3 mcg/g) from the sample by using UV spectrophotometer at 513 nm.