Effect of giloy (Tinospora cordifolia) supplementation to semen extender on selected quality parameters of magra ram semen

Abstract

To investigate the impact of antioxidant potential on several seminal parameters of Magra ram semen during chilled storage at 4 °C, ethanolic extract of Tinospora cordifolia (Giloy) was added at concentrations of 100 µg/ml, 300 µg/ml, 500 µg/ml, and 700 µg/ml to ovine semen extender. Tris egg yolk citrate fructose extender (TEYCF), which is aided with various quantities of T. cordifolia extract, served as the basic extender. Findings showed that, mean values for percentage of individual sperm motility and live sperm percentage were significantly (p<0.05) higher in all treatment groups as compared to control at 24, 48, 72 and 96 hrs of storage. At 48, 72 and 96 hrs, the mean values of hypo-osmotic swelling test positive spermatozoa percentage were noted significantly (p<0.05) higher in all treatment groups as compared to control. It was concluded that the quality of liquid semen stored at 4 °C was enhanced by the addition of T. cordifolia extract at varying quantities in semen extender. This enhancement may have been caused by T. cordifolia's antioxidative properties. Maximum effects were obtained with 300 µg/ml of T. cordifolia extract concentration.