Synthesis, characterization and efficacy of green synthesized copper nanoparticles from eucalyptus, against Fusarium wilt of green gram

Abstract

Green gram suffers heavy losses in yield due to many diseases especially wilt caused by Fusarium sp. Thus, the present study was undertaken with emphasis on development of simple and cost-effective methods for synthesis of nanomaterials and their application in the management of the pathogen. Green synthesis methods, employing plant extracts, have emerged as an eco-friendly and clean alternative to physical and chemical methods. The eucalyptus plant extract mediated copper nanoparticles were synthesized using 0.01M of CuSO4 solutions and characterized by usingUV-Visible spectrophotometry, Zeta size analysis, Zeta potential, and Nano Tracking Analysis. The synthesized nanoparticle (129.23 nm) were applied at various concentrations to determine their antifungal activities. The result showed the highest inhibition percentage of mycelial growth (80.59%) was recorded on CuNP-E @ 1000 ppm.