Oocyte yield and oocyte quality following FSH stimulation in Sahiwal cattle

Abstract

Ultrasound guided transvaginal ovum pick up and In-Vitro Embryo Production has opened up greater possibilities to exploit productive and reproductive potential of the superior cows. In the present study, ten Sahiwal donor cows were pre-stimulated with multiple injections of FSH prior to OPU. Total 27 OPU sessions were conducted of which 602 oocytes were recovered with an average yield of 22.37 ± 2.01 oocytes per each session of OPU. Aspirated oocytes were graded as grade A, grade B, grade C, grade D and grade E oocytes (10.78 ± 1.20, 2.93 ± 0.41, 3.00 ± 0.33, 5.26 ± 0.88 and 0.33 ± 0.15 respectively). Of the 602 COCs recovered 451 were viable (74.92%) and 151 were non-viable (25.08%). Of all the grades of oocytes the difference in the quality of Grade A and with the other Grade oocytes was significant (p