Cladosporium fungi isolated from Bali Dog’s skin

Abstract

Fungal diseases in animals are often ignored, but the economic losses due to fungal infections are quite disturbing, damage to skin, fur and the potential for zoonoses need to be considered. A lot of fungi are widespread in the environment but many of their species are not well understood especially their roles in human and animal health. This research aims to study further about the identification process using morphology and molecular method of Cladosporium fungi that found in dog’s skin. The sample for this study was a fungal isolate from the skin of a Bali dog which was suspected to be infected with a fungus. Identification is carried out by microscopic and macroscopic observations using Saboraud Dextrose Agar (SDA) media. The molecular methods are using the PCR ITS1 and ITS4 primers and GeneJET PCR Purification Kit. The result showed that 30% of the samples are identified as Cladosporium fungi, with the specific species is Cladosporium tenuissimum.