Callus induction through different explants in alphonso mango (Mangifera indica L.)

Abstract

A Laboratory experiment was conducted during year 2022-2023 at Plant Biotechnology Centre, College of Agriculture, Dapoli, Dr. B. S. Konkan Krishi Vidyapeeth, Dapoli to callus induction by using different explant and in Alphonso Mango (Mangifera indica L.). The four different sources of explant viz., Apical buds, Auxiliary bud, Nucellar and leaf were used with MS media. This experiment imposed that the callus induction in mango is depend upon the quantity of 2,4-D and NAA present in the nutrient media. The result revealed that apical bud and axillary bud media combination AM3- (MS+ 2 mgl-1 2, 4 - D + 1 mgl-1 NAA) founded superior. In case of nucellar tissue media combination AM7- (MS+2.5 mgl-1 2, 4 - D + 1 mgl-1 NAA) gave the best result. In respect to leaf explant highest success was observed in media combination AM7- (MS+2.5 mgl-1 2, 4 - D + 1 mgl-1 NAA) as compare to other three explants.