Superovulatory response and embryo recovery in Kankrej cattle

Abstract

Superovulation is a reproductive technology used in the dairy industry to enhance the reproductive rate of superior female animals. The experiment was conducted to investigate the superovulatory response and embryo recovery in twelve donor Kankrej cattle which were divided in to two groups (A and B) and synchronized with single dose (625 μg, im) of cloprostenol. Superovulation was induced with pFSH (25 mg) in tapering dose started on 8th day of estrus for 4 days. Group-A cows (n=6) were given 10 µg GnRH While, Group-B cows (n=6) did not receive GnRH at the time of breeding. Estrus following superovulation (53±2.15) took less time in hours than induced estrus after synchronization (61.2±5.01). Of the 12 cows, 8 (66.67%) were responding to the superovulation treatment (>2 CL), while 4 (33.33%) were not responding (≤ 2 CL). Using rectal palpation and ultrasonography, total mean CLs were found in Group A to be 4.33±1.27 and 5.67±1.62, and in Group B to be 3.16±.94 and 4.17±.74 respectively. Furthermore, compared to the number of CLs in Group B, there was a substantial (p