Genetic exploration of dwarf traits in red banana mutants: Integrating molecular markers for sustainable cultivation

Abstract

This study set out to investigate red banana mutants in-depth and used a careful molecular analysis to determine the genetic cause of dwarfism. The study, which used 3,200 red banana plants grown In vitro and exposed to various gamma irradiation dosages, determined that an LD50 dose of 20 Gy was necessary for the best possible growth and survival. The use of Sequence Characterized Amplified Regions markers in DNA analysis revealed the presence of the dwarf gene in select samples, with Sample No. 1 leading to notable findings and subsequent identification of specific dwarf plants Samples 14, 18, and 52 at both group and individual plant levels. This research is important because it emphasizes the integration of morphological observations with molecular investigation, going beyond genetic discoveries. The study's strength lies in the convergence of molecular markers with morphological and biochemical analyses, enhancing the overall validity of its findings. Considering these findings, the thorough molecular screening serves to illuminate the wider implications for breeding programs as well as the genetic characteristics associated with dwarfism in red banana mutants. In essence, this research bridges the gap between genetic insights and practical applications, paving the way for sustainable crop development and enhancing the adaptability of red banana cultivation in diverse environmental conditions.