Analysing IL8, IL10, IFNɑ and IFNγ levels in pregnancy and pathological conditions in Karan Fries

Abstract

The maternal immune system has immune tolerance to fetus so that it is not rejected. This immune tolerance involves cytokines produced by T cells. This study was conducted to monitor various pro- and anti-inflammatory cytokines in both pregnant cows as well as diseased cows. Cows suffering from clinical mastitis and endometritis were selected for the study. In pregnant cows, blood sample was collected on day 10, 18, 36 post artificial insemination. Plasma of both pregnant and diseased cows was isolated and IL8, IL10, IFNÉ‘, and IFNγ levels were estimated. IL10 levels were up regulated in pregnant animals. IL8 levels were higher in pregnant cows. IFNÉ‘ and IFNγ levels were higher both in pregnant and diseased cows.