Study of prevalence and isolation of pathogens in sub clinical mastitis

Abstract

Two hundred quarter milk samples from fifty seemingly healthy cows with varying parities were tested using the California Mastitis test and culture analysis. On a quarterly basis, the prevalence determined by CMT was 36% (72/200) and determined by culture analysis, 30% (60/200). According to CMT and a culture examination, the prevalence on an animal basis was forty (20/50) and forty percent (23/50). The highest prevalence was seen in the IV th parity on a quarterly basis and in the V th parity on a cow basis. 50.56% of the Staphylococcus aureus organism was determined to be predominant in this study based on culture testing. The major pathogen was identified as Staphylococcus spp. (42%), which was isolated by bacterial mPCR.