Investigating antibacterial potential of hippasa spider silk

Abstract

Present study was designed with an aim to evaluate anti-bacterial potential of spider silk of Hippasa holmerae against gram negative bacteria E. coli. Extracts of spider silk were prepared in three different solvent – ethanol, methanol and acetone; and antimicrobial activity was carried out. Spider silk inhibited the growth of the E. coli. Effective zone of inhibition against the bacteria E. coli for the Hippasa holmerae silk extracts were observed as- in Methanol extract 10 mm zone of inhibition, Ethanol extract have 8 mm zone of inhibition and for Acetone extract 12 mm of inhibition after 24 hours. Whereas Methanol extract showed 10 mm zone of inhibition, Ethanol extract 9 mm zone of inhibition and Acetone extract 13 mm zone of inhibition after 48 hours. Amoxicillin reactivity was observed for E. coli as 9 mm and 10 mm zone of inhibition after 24 and 48 hours respectively.