Frequencies and phenotypes of antibiotic resistance in non-fermentating bacteria isolated at the China-Guinea Friendship Hospital of Kipé in Conakry, Guinea

Abstract

Introduction: Multidrug-resistant bacterial infections constitute a major global public health problem, particularly in developing countries. Objective: To determine the diversity and antibiotic resistance phenotypes of non-fermentative Gram negative bacilli (NFGNB) isolated at the China-Guinea Friendship Hospital (HASIGUI). Methodology: This is a nine-month prospective study, carried out in patients at the HASIGUI Biomedical Laboratory from May 1, 2019 to February 1, 2020. Bacterial identification and antibiogram were carried out using a machine. Vitek 2 Compact. Results: A total of 86 NFGNB strains isolated from different biological fluids were analyzed. The average age of the patients was 45.08 years with the range from 1 year to 98 years. The female gender was dominant with 59.30%. The sex ratio (M/F) was 0.6. Fourteen NFGNB species belonging to seven bacterial genera were identified. Sphingomonas paucimobilis was the predominant species (33.72%), followed by Acinetobacter baumanni complex (22.10%), Pseudomonas aeruginosa and Pseudomonas fluorescens each representing 10.46%. Cases of multi-resistance were observed in all families of antibiotics tested. Resistance was common in some antibiotics: oxacillin (100%), ampicillin (62%), norfloxacin (66%), nalidixic acid (66%), cotrimoxazole (59.42%), cefoxitin (50%), and cefuroxime (50%). On the other hand, the most active antibiotics on BGNnf were: vignecycline (100%), imipenem (97.23%), meropenem (88.24%), minocycline (85%), piperacillin-tazobactam (83.87%), amikacin (93.87%), gentamicin (84%), tobramycin (81.43%), levofloxacin (64.28%), ofloxacin (61.70%), ciprofloxacin (60%). Conclusion: Antibiotic resistance phenotypes of BGNnf indicate the need for antibiotic therapy based on antibiogram results.