Bio-Chemical evaluation of fresh Kangayam Bull Semen

Abstract

Evaluation of the semen is necessary to assess the bull fertility. For the accuracy in the result of semen evaluation strict quality control measures should be applied. The current necessity is the rapid proliferation of native cow breeds, particularly Kangayam cows, via artificial insemination using high-grade semen. For the investigation, 5-7-year-old Kangayam bull Nos. NK 16, NK 17 and NK 30 were used. The semen was collected twice in a week and two ejaculates in each time were collected. They were subsequently transported immediately to the laboratory for processing semen and maintained in a water bath at 34° C. After the primary screening of semen through microscopical analysis the semen samples were subjected to biochemical analysis. From the biochemical analysis of bull semen out of 24 samples, 20 samples showed a pH of 6.7 and 4 samples with 6.8. The mean (± SE) methylene blue dye reduction time (MBRT) of fresh semen samples was 4.20±0.01 minutes. The mean fructolytic index of fresh semen samples was 1.02±0.37. This study shows the significance in evaluating the biochemical characteristic of the fresh Kangayam bull semen.