Gas Chromatography-Mass Spectrometry and In vitro Ethnopharmacological Investigations of the Solvent Ethyl Acetate Extract of Urtica dioica L. Leaf from Wokha, Nagaland, India

Abstract

Urtica dioica L, a plant species belonging to the Urticaceae family, has been documented from the Wokha district of Nagaland, India. The highest level of antibacterial activity observed in the solvent ethyl acetate extracted plant with regards to the zone of inhibition was 17.33.±1.52, which was exhibited against Salmonella typhimurium at 100 μg/mL concentration. The potent IC50 values in ABTS assay was found to be 23.69±0.32 µg/mL for the ethyl acetate extract. Similarly, the IC50 values for the HRSA assay were determined to be 24.66±0.38 µg/mL in ethyl acetate extract. The solvent extract of ethyl acetate exhibited the IC50 values for α-glucosidase, measuring 16.60±0.63. A Gas Chromatography-Mass Spectrometry study was conducted to identify the component present in the solvent ethyl acetate leaf extract of Urtica dioica. A total of thirty bioactive phytochemical components were detected in the ethyl acetate extract of Urtica dioica. This findings may potentially facilitate the creation of innovative pharmaceutical agents.