High-resolution melting analysis of chromodomain helicase DNA binding protein 9 (CHD9) gene in goat breeds of Kerala, India

Abstract

The objective of this study was to identify polymorphisms in chromodomain helicase DNA binding protein 9 (CHD9) gene and assess its potential impact on body weight and conformation traits within the Malabari and Attappady Black goat breeds in Kerala. Significant differences were observed in adult body weights, height at withers and chest girths between the Malabari and Attappady Black breeds. Genotyping using High-Resolution Melting (HRM) revealed AA, GG and AG genotypes of CHD9. The highest frequency of AA genotype was observed in both breeds. Both Malabari and Attappady black breeds were in Hardy-Weinberg equilibrium. Notably, the genotypic patterns of CHD9 did not exert a significant effect on body weight or other conformation traits in either breed.