Hybridity confirmation in F1s of Indian mustard (Brassica juncea Czern & Coss) by integrating microsatellite markers and disease reaction

Abstract

Confirmation of true F1 hybridity in Indian Mustard is a crucial step in the selection of positive segregating individuals after the hybridization program in crop breeding. The use of molecular markers is the most reliable and rapid method rather than the grow-out test (GOT). The present study aimed at the assessment of genetic purity of the generated F1 plants in Indian Mustard (Brassica juncea) using molecular markers as well as visual disease reaction as PDI. Out of 80 SSR markers used for the parental polymorphism study, 18 were found to be polymorphic between the parents Varuna and CAULC-2 (Locally collected from Kakching wairi, Manipur and highly tolerant o Albugo candida). Among polymorphic markers obtained from two parental line, the markers At2g36360 could identify the true F1 plants. The plants that showed lower PDI were selected for further development of segregating or mapping population. This study showed that these SSR markers may be used as referral SSR markers for testing hybridity and genetic purity in Brassica breeding programs.