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Abstract

Background: The aim of this research was to explore how both aerobic exercise and resveratrol supplementation impact the antioxidant enzymes and immune function of skeletal muscle in mice.Methods: A total of four groups were established, consisting of six-week-old male ICR mice: 1) The CON group (Control, n=7), 2) The RE group (Resveratrol supplementation, n=7), 3) The EX group (Aerobic exercise, n=7), 4) The EX+RE group (Aerobic exercise and resveratrol supplementation, n=7).Aerobic exercise was conducted on a treadmill at a speed of 15 meters per minute (corresponding to 60% of their maximum oxygen consumption), with a 0-degree incline.This exercise regimen was carried out five days a week for a duration of six weeks.Additionally, resveratrol was administered at a dosage of 25mg per kilogram of body weight once a day, five days a week, for a total of six weeks.To assess the levels of specific markers in the gastrocnemius muscle, such as SOD (Superoxide Dismutase), CAT (Catalase), TNF-1 (Tumor Necrosis Factor-1), and IL-6 (Interleukin-6), Western blotting analysis was employed.Results: The expressions of SOD 1 and 2 were higher in all groups compared with CON (p<0.05 for all).EX and EX+RE were higher than RE (p<0.05).While no difference in SOD 1 was detected between EX and EX+RE, SOD 2 was lower in EX+RE relative to EX (p<0.05).CAT were higher in EX and EX+RE than CON or RE (p<0.05 for all).Interestingly, CAT was greater in EX+RE than EX (p<0.05).The expressions of TNF-1 and IL-6 in all groups were significantly lower than CON group (p<0.05 for all).TNF-1 in EX+RE group was significantly lower than RE and EX groups (p<0.05).IL-6 in EX+RE group was significantly lower than RE and EX groups.Conclusions: Co-treatment of aerobic exercise and resveratrol supplementation have a positive synergistic effect for antioxidant enzymes and immune function.