Isolation, structural characterization and evaluation of the in vitro antioxidant potential of four compounds isolated from the selective leaf extracts of Bauhinia monandra Kurz (Fabaceae)

Abstract

Bauhinia monandra belongs to Fabaceae family, is used in traditional Ivorian medicine to treat diabetes and male infertility. A preliminary study conducted on the leaves, stems and roots showed fluctuating contents of total phytophenols and flavonoids in the ethyl acetate extract of the leaves. This study made it possible to isolate and elucidate the molecular structures of four phytoconstituents from selective leaf extracts: Vomifoliol (1), guibourtinidol (2), epiafzelechin (3) and D-pinitol (4). The molecular structures of the phytoconstituents were elucidated by NMR 1H, 13C, COSY, HMBC et HMQC. Also, There in vitro antioxidant activity was measured using the DPPH and FRAP spectrophotometric methods.