Use of ovaprim and Chorulon hormones for spawning induction of G4 transgenic Mutiara catfish Brood stock in rearing indoor Hatchery

Abstract

This research was conducted at Hatchery Building 4, the Fisheries Biotechnology and Marine Sciences Laboratory of Padjadjaran University from October to November 2022, aims to obtain the optimal combination dosage of ovaprim and HCG hormones on the reproductive performance of mutiara catfish. The study used a completely randomized design (CRD) with four treatments (pair A: 0.4 ml ovaprim + 0.6 ml HCG, B: 0.5 ml ovaprim + 0.5 ml HCG, C: 0.6 ml ovaprim + 0.4 ml HCG for transgenic fish pairs, and D: 0.4 ml ovaprim + 0.6 ml HCG for non-transgenic fish pairs) with three pairs of broodstock as replicates. The research results showed that the best dosage to induce spawning in transgenic G4 mutiara catfish was found in treatment B (0.5 ml ovaprim/kg female and male broodstock weight and 0.5 ml HCG/kg male broodstock weight). After spawning, the broodstock pairs in treatment B showed the best results with an average relative fecundity of 85,103 eggs/kg broodstock, fertilization rate (FR) reaching 88.150%, hatching rate (HR) reaching 90.056%, and survival rate (SR) reaching 88.034%.