Effect of growth regulators on shoot proliferation and root development in micropropagation of pomegranate (Punica granatum L.)

Abstract

In this study, the effect of growth regulators in different concentrations was tested for shoot and root development in micropropagation of pomegranate with nodal explants. The explants were collected in the morning, third node from the shoot tip. The explants were collected from healthy plants which were authenticated. For shoot development The MS media was supplemented with different concentrations of alpha-naphthalene acetic acid (NAA), indole-3-butyric-acid (IBA), 6-benzyl aminopurine (BAP) and kinetin and their combinations. For rot development MS media was supplemented with different concentrations of alpha-naphthalene acetic acid (NAA), indole-3-butyric-acid (IBA) was tested. It is observed that MS media when supplemented with ADS 15 mg/l + NAA 2.0 mg/l + BAP 2.0 mg/l had given highest maximum shoot proliferation, number of shoots, number of leaves along with highest number of nodes and intermodal length and survival per cent, followed by MS media supplemented with ADS 15 mg/l + NAA 1.0 mg/l + BAP 2.0 mg/l. MS media when supplemented with IBA 0.6 mg/l had given a maximum number of roots per shoot, highest number of shoots producing roots and more number of new leaves followed by MS media supplemented with 0.6 mg/l NAA.