Evaluation of local solid and liquid substrates for growth and sporulation of Trichoderma asperellum

Abstract

Rotten maize grain, maize stone and banana pulp supplemented with jaggery were evaluated as substrates for the growth and sporulation of Trichoderma asperellum. Significant increased sporulation per gram was observed in jaggery added substrate. Among solid substrates, significant maximum growth and sporulation per gram was observed in Sorghum grain+2% Jaggery (8.31x108) followed by Maize grain+2% Jaggery (5.54x108) and Sorghum grain (5.24x108). Different concentrations (2, 4, and 6%) of Banana pulp in water was also evaluated and maximum sporulation (spores/ ml) was observed in potato pulp+ 2%Jaggery (10.20x107) followed by Banana pulp (6%) + 2%Jaggery (9.72x107) and was at par with Banana pulp (4%)+2%Jaggery. Minimum sporulation was observed with Banana pulp (2%) + (3.45x107). The agriculture waste like rotten maize and banana supplemented with jaggery may be utilized for the mass production of Trichoderma asperellum bioagent.