Effect of pregnancy on retrieval of oocytes for IVF in Deccani ewes

Abstract

A total number of 76 gravid genitalia of Deccani breed of ewes was procured from local abattoirs. The morphometrics of the ovaries of gravid genitalia were recorded. The numbers of visible follicles on right and left ovaries were 1.81±0.14 and 1.75±0.14 irrespective sizes of follicle. The mean number of oocytes retrieved from ovaries during different periods of gestation was 7.83±0.63 up to 30 days, 8.27±0.70 in 31-60 days, and 9.50±0.70 in 61-90 days and 9.05±0.70 in 91-120 days of gestation, respectively. The mean number of oocytes retrieved in aspiration, dissection and slicing techniques were 9.33±0.76, 9.41±0.36 and 7.25±0.50 in ovaries collected from gravid genitalia. Significantly, longer time was required to process the ovary by dissection technique than slicing and aspiration techniques.