Standardization of in vitro organogenesis technique in bamboo (Dendrocalamus stocksii)

Abstract

Sterilized explants were inoculated on MS media containing different concentrations and combinations of cytokinins (BAP, BAP+TDZ) 90% bud break was obtained in the MS medium supplemented with 1.0 mg/l BAP and 0.5 mg/l TDZ. The MS medium with 1.5 mg/l BAP and 1.0 mg/l TDZ had the highest average of 10 shoots/explant and a shoot length of 3 cm. The highest shoot multiplication was obtained with a clump of 9 shoots on MS medium containing 1.0 mg/l BAP and showed 39 shoots/culture with the highest average shoot length of 6.00 cm. A clump of 4-5 shoots were subculture for root induction on MS medium containing different concentrations of IBA and NAA. The combination of MS Medium with IBA 0.5 mg/l + NAA 1.5 mg/l resulted in 67.21% rooting and 7.80 roots/explant. The medium with 35 gm/l sucrose shoots had an average length of 6.83 cm, a 4.5 cm length of roots, and 8 roots. So, it’s considered the optimum concentration of sucrose. Rooted plants were transferred to a mixture of soil: cocopeat: FYM The maximum number of plants that survived was 56.77% in a 1: 1: 1 ratio of mixture.