Cloning and sequencing of lipoprotein signal peptidase (LSP) gene of Anaplasma marginale

Abstract

An enzyme known as Lipoprotein signal peptidase (LSP), is essential for the production of lipoproteins. LSP is responsible for cleaving the signal peptide from preprolipoproteins, allowing their proper targeting and insertion into the cell membrane. The sequence of the peptidase was inferred from the nucleotide sequence of the Anaplasma marginale LSP gene, whice codes for lipoprotein signal peptidase. The effectiveness of lipoprotein signal peptidase (LSP) inhibitor, against the Anaplasma genus of Gram-negative bacteria is demonstrated. The study chooses to target LSP, the enzyme in charge of digesting lipoproteins and determine the effect on Anaplasma survivability. Basically the truncated LSP gene of Anaplasma marginale is cloned and sequenced in this current study.