Synthesis, characterization, in silico prediction and safety evaluation of topical wound healing formulations of hemin and bilirubin nanoparticles

Abstract

Nanotechnology is an emerging scientific area of research interest. A number of nanomedicine-based therapeutic agents have been synthesized for different ailments. However, safety evaluation of nanoparticles is still a major issue. Present research reports the synthesis of hemin nanoparticles (HNPs) & bilirubin nanoparticles (BNPs) as topical formulations for wound healing based on the information available regarding the pharmacological effects of hemin and bilirubin. In silico prediction of hemin and bilirubin revealed that both compounds have high lipophilicity, low water solubility and skin permeation. Hence, HNPs and BNPs were synthesized to overcome these hindrances. Furthermore, safety evaluation of topical formulations of HNPs and BNPs applied for 19 days in diabetic rat wound model was done in terms of haematology and gross necropsy which revealed that HNPs and BNPs treated groups showed no alteration in haematology and no pathological changes in the vital organs were observed.